zymolyase® , produced by a submerged culture of arthrobacter luteus , has p lytic activity against living yeast cell walls to produce protoplast or spheroplast of various strains of yeast cells. an essential enzyme for the lytic activity of zymolyase® is β-1,3-glucan laminaripentaohydrolase. it hydrolyzes linear glucose polymers with β-1,3-linkages and releases specifically laminaripentaose as the main and minimum product unit . there are two preparations of zymolyase® , zymolyase® -20t and zymolyase® -100t, having lytic activity of 20,000 units/g and 100,000 units/g respectively. zymolyase® -20t is ammonium sulfate precipitate while zymolyase® -100t is a further purified preparation by affinity chromatography .lytic activity varies depending on yeast strain, growth stage of yeast, or cultural conditions.further informations related to zymolyase® can be obtained in the reference section below .