Zymolyase® , produced by a submerged culture of Arthrobacter luteus , has strong lytic activity against living yeast cell walls to produce protoplast or spheroplast of various strains of yeast cells. An essential enzyme for the lytic activity of Zymolyase® is β-1,3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers with β-1,3-linkages and releases specifically laminaripentaose as the main and minimum product unit . There are two preparations of Zymolyase® , Zymolyase® -20T and Zymolyase® -100T, having lytic activity of 20,000 units/g and 100,000 units/g respectively. Zymolyase® -20T is ammonium sulfate precipitate while Zymolyase® -100T is a further purified preparation by affinity chromatography .Lytic activity varies depending on yeast strain, growth stage of yeast, or cultural conditions.Further informations related to Zymolyase® can be obtained in the reference section below .