Type of coating | Immobilization method | Typical probes | Ordering information | |||
NEXTERION®product | Barcode option | Item number | Slides per pack | |||
Aldehydesilane 2-D surface | Amine reactive chemistry Covalent binding | Amino-modified PCR products, BACs and oligonucleotides | Slide AL | None | 1064874 | 25 |
Laser | 1064876 | 25 |
Key product features |
• Stable and covalent binding of probes such as amino-modified nucleic acids, cells, and peptides |
• Optimal accessibility of probes through specific end-point attachment |
Typical applications |
• Array CGH using whole genome tiling path BAC arrays |
• miRNA expression profiling |
• Indel oligonucleotide arrays for genotyping |
• 16S rRNA-based taxonomic microarray |
• Protein-DNA interaction studies |
• Protein-protein interaction studies |
Suitable probe types |
• Amine-PCR products and BACs |
• Amino-modified oligonucleotides |
• Small protein fragments such as peptides |
• Cells and tissues |
Protocol step | Recommended NEXTERION®products | Alternatives | Additional information |
Spotting | NEXTERION® Spot + detergents like cetyltrimethylammoniumbromid, Triton X-100, sarcosyl, Tween or SDS with a final concentration between 0.005 and 0.05 % (to increase the spot size) | Recommended Spotting Concentrations: PCR products: 0.1 – 1 µg/µL Oligonucleotides: 10 – 20 µM Peptides: 100 – 500 µg/mL Recommended Spotting Conditions: Constant relative humidity 40 – 50% at 20 – 24°C (68 to 75°F). Spotting solutions may be used with, or without protein-stabilizing agents | |
3x SSC | |||
3x SSC + 1.5 M betaine (low evaporation buffer for long spotting runs) | |||
Chemical deactivation | - | 1.0 g NaBH4 in 300 mL PBS and 100 mL ethanol | |
Hybridization | 3 – 5x SSC + 0.1 % SDS | Add competitor DNA if appropriate |
Process step | SCHOTT product | Quantity |
Spotting | NEXTERION® Slide AL | 10 slides |
NEXTERION® Spot | 10 mL | |
Hybridization | NEXTERION® Hyb | 10 mL |
Oligo test probe | 2 nmol | |
Oligo test target | 3 pmol |