找化学品上960化工网!
960化工网
提取细菌基因组dna除了CTAB/NaCl 法之外还有其他方法吗?谢谢!
具体步骤有哪些谢谢。我自己去知网查吧。不用回答了... 具体步骤有哪些谢谢。我自己去知网查吧。不用回答了 展开
网友 1

最佳答案

回答者:网友
有,用试剂盒。
独特的结合液/蛋白酶K迅速裂解细胞和灭活细胞内核酸酶,然后基因组DNA在高离序盐状态下选择性吸附于离心柱内硅基质膜, 再通过一系列快速的漂洗-离心的步骤,抑制物去除液和漂洗液将细胞代谢物,蛋白等杂质去除, 最后低盐长欢丝至力育省比的洗脱缓冲液将纯净基因组DNA从硅基质膜上洗脱。

特点:
1.离心吸附柱内硅基质膜全部采用进口世界著名公司特制吸附膜,柱与柱之间吸附量差异极小,可重复性好。克服了国产试剂盒膜质量360问答不稳定的弊端。
2绿.不需要使用有毒的苯酚等试剂,也不需要乙醇沉淀等步骤
3.快速,简捷,单个样品操作一般可在30分钟内完成。
4.多次柱漂洗确保高纯度,OD260/OD280典型的比值达1.7~1.9,长度可达30kb -50kb,可直接用于PCR,Southern-blot和各种酶切反应。

发表的部分文章:

1.Vibrio zhanjiangensis sp. nov., isolated from sea water 觉口留相会还常境眼续石of shrimp far绿作免钱算孙干春业ming pond. Antonie va罪直杀波下变细n Leeuwenhoek, 2011, Published online: 29 December 20普革氧策倍语轴冷11
2.Research on resusci笔题tation of viable but non-culturable state E.coli O157:H7. Food Scienc子马厚析测会卷危e, 2012, Online Ahead of Print弦当众氧查造困: Dec 11, 2012
3.Characterization of marine Pseudomonas spp. a行委维稳ntagonist towar施型治ds three tuber-rotting fungi 环西心证着顶from Jerusalem artichoke, a new industrial crop. Industrial Crops and Products, 2013, 43:556–561
4.Azospirillum humicireducens sp. no刘示v., a nitrogen-fixing bacterium isolated from a microbial fuel cell. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2012, Published online ahead of print December 21, 2012,
5.Vibrio zhuhaiensi市喜行政进识土迫达续s sp. nov., isolated f土没脱范源rom a Japanese prawn (Marsupenaeus japonicus). Antonie van Leeuwenhoek, 2013, Published online: 22 January 2013 as DOI 10.1007/s10482-013-9878-4
6.Bacillus composti sp. nov. and Bacillus thermophilus 1 sp. nov., two thermophilic 2 Fe(III)-reducing bacteria isolated from a compost. IJSEM, 2013, Published February 8, 2013 as DOI:10.1099/ijs.0.049106-0
7.Bacillus thermocopriae sp. nov., 1 isolated from a compost. IJSEM, 2013,Published February 8, 2013 as doi:10.1099/ijs.0.046953-0
8.Resuscitation of Viable but Non-culturable E. coli O157:H7. 218 2013, Vol.34, No.11
9.Induction and PMA-qPCR Detection of Listeria monocytogenes in Viable but Non-Culturable State. Science paper Online
10. DETERMINATION OF DNA-DNA RELATEDNESS IN VIBRIOS BY MEANS OF REAL-TIME PCR. ACTA HYDROBIOLOGICA SINICA. Vol.37,No.4,Jul.2013
11. Kroppenstedtia guangzhouensis sp. nov., a thermoactinomycete 1 isolated from soil.IJSEM Papers in Press. Published May 31, 2013 as doi:10.1099/ijs.0.051011-0
12. Bacillus sediminis sp. nov., isolated from an electroactive biofilm. Antonie van Leeuwenhoek, DOI 10.1007/s10482-013-0032-0
13. Bacillus borbori sp. Nov., Isolated From an Electrochemically Active Biofilm. Curr Microbiol (2013) 67:718–724, DOI 10.1007/s00284-013-0426-2
14. Bacillus thermotolerans sp. nov., a thermophilic bacterium c 1 apable of reducing humus. IJSEM Papers in Press. Published April 26, 2013 as doi:10.1099/ijs.0.048942-0
15. Pseudomonas guangdongensis sp. nov., isolated 1 from an electroactive biofilm, and amended description of the genus Pseudomonas Migula 1894. IJSEM Papers in Press. Published August 5, 2013 as doi:10.1099/ijs.0.054676-0
16. Azospirillum humicireducens sp. nov., a nitrogen-fixing bacterium isolated from a microbial fuel cell. IJSEM Papers in Press. Published December 21, 2012 as doi:10.1099/ijs.0.046813-0
17. Resuscitation of Viable but Non-culturable E. coli O157:H7. Food science, 218 2013, Vol.34, No.11
18. Identification of lactic acid bacteria in commercial yogurt and their antibiotic resistance. Aicrobiologica Sinica 53(8):889-897;4 August 2013
19. Sinibacillus soli gen. nov., sp. nov., a moderately thermotolerant member of the family Bacillaceae. IJSEM Papers in Press. Published February 7, 2014 as doi:10.1099/ijs.0.055608-0.
20. Design and Properties Validation of Thiazole Orange Monoazide-A Nucleic Acid Crosslinker Based on the Activity of Esterase in Bacteria.
21. Effects of CagA+ Helicobacter pylori infection on expression of HIF-2α and ABCG2 in human gastric cancer cell line SGC7901 under normoxia and hypoxia conditions. ISSN 1009-3079 (print) ISSN 2219-2859 (online)

我来回答

平台客服 平台客服

平台在线客服